Table of Contents
What kind of virus is VSV?
Indiana vesiculovirus, formerly Vesicular stomatitis Indiana virus (VSIV or VSV) is a virus in the family Rhabdoviridae; the well-known Rabies lyssavirus belongs to the same family. …
What is VSV disease?
Vesicular stomatitis is a viral disease which primarily affects horses, cattle, and swine. The agent that causes vesicular stomatitis, VSV, has a wide host range and can occasionally infect sheep and goats.
How is VSV classified?
VSVs are classified in the order Mononegavirales (non-segmented-negative-sense RNA viruses), family Rhabdoviridae, genus Vesiculovirus.
What is the shape of Rhabdovirus?
They have complex bacilliform or bullet-shaped. All these viruses have structural similarities and have been classified as a single family. The virions are about 75 nm wide and 180 nm long. Rhabdoviruses are enveloped and have helical nucleocapsids and their genomes are linear, around 1115 kb in length.
Is VSV a DNA virus?
VSV is another single-stranded RNA virus of the rhabdoviridae family, which includes the rabies virus.
How do you treat VSV?
There are no specific antiviral agents. Treatment consists of supportive care including the prevention or treatment of secondary infections. A mild antiseptic mouthwash may reduce the pain associated with oral lesions. Nutritional support is provided if eating is impaired.
Can stomatitis be transmitted to humans?
Humans can contract vesicular stomatitis by coming into contact with lesions, saliva, or nasal secretions from infected animals. In people, the disease causes an acute influenza- like illness with symptoms such as fever, muscle aches, headache, and malaise.
Is there a vaccine for vesicular stomatitis?
We have previously developed a fast-acting and efficacious vaccine against Ebola virus (EBOV) using the vesicular stomatitis virus (VSV) platform.
What cells do VSV infect?
VSV infects a broad range of animals, including cattle, horses, and swine. The genome of the virus codes for five major proteins, glycoprotein (G), matrix protein (M), nucleoprotein (N), large protein (L), and phosphoprotein (P).
Can humans get VSV?
Yes. Humans can become infected with VSV when handling infected ani- mals (direct contact). In affected people, vesicular sto- matitis causes a flu-like illness with symptoms of fever, muscle aches, headache and weakness. Rarely, humans can get oral blisters similar to cold sores.
What is VSV G protein?
Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.
What is equine vesicular stomatitis?
Vesicular Stomatitis (VS) is a contagious disease that afflicts horses, livestock, wildlife and even humans. The disease is caused by a virus, which although rarely life threatening, can have significant financial impact on the horse industry.
What does pox virus cause?
Poxviruses exist throughout the world and cause disease in humans and many other types of animals. Poxvirus infections typically result in the formation of lesions, skin nodules, or disseminated rash. Infection in humans usually occurs due to contact with contaminated animals, people, or materials.
Is Rhabdovirus a DNA virus?
The family Rhabdoviridae consists of mostly enveloped, bullet-shaped or bacilliform viruses with a negative-sense, single-stranded RNA genome that infect vertebrates, invertebrates or plants.
What is the size of pox virus?
Poxviruses are brick-shaped (240 nm by 300 nm) and have a complex internal structure including a double-stranded DNA genome (130260 kb) and associated enzymes. Naturally released virions have an additional outer membrane not found on infective virions extracted artificially from infected cells.
Is VSV an adenovirus?
Three recombinant adenoviruses (rAd) were successfully constructed that expressed the VSV Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein (both serotypes of G [VSV-IN-G-NJ-G]) with potentiality to induce protective immunity.
What does VSV G bind?
VSV genome encodes five structural proteins among which a single-transmembrane glycoprotein (G). G plays a critical role during the initial steps of virus infection4. First, it is responsible for virus attachment to specific receptors. After binding, virions enter the cell by a clathrin-mediated endocytic pathway5 , 6.
What is vesicular stomatitis virus glycoprotein?
Abstract. The glycoproteins (G proteins) of vesicular stomatitis virus (VSV) and related rhabdoviruses (e.g., rabies virus) mediate both cell attachment and membrane fusion. The reversibility of their fusogenic conformational transitions differentiates them from many other low-pH-induced viral fusion proteins.
What is the cause of vesicular stomatitis?
Vesicular stomatitis is caused by a virus and affects horses, cattle, and pigs. It also rarely affects sheep, goats, and llamas. The virus can be transmitted to humans and may cause flu-like disease. Vesicular stomatitis is seen only sporadically in the US.
Is VSV reportable?
VS is a state reportable disease.
What states have vesicular stomatitis?
Vesicular stomatitis virus (VSV), a reportable animal disease, has been found in seven states in 2020: Arizona, Kansas, Missouri, Nebraska, New Mexico, Oklahoma and Texas. As VSV may spread throughout the Midwest and mountain states, livestock owners, especially those with horses, should be looking for symptoms.
What does stomatitis look like?
Aphthous stomatitis are round or oval ulcers with a red, inflamed border. The center is usually white or yellow. Most canker sores are small and oval, and heal within one to two weeks without scarring.
What is the most common cause of stomatitis?
Often it will be due to injury, infection, allergy, or skin disease. Share on Pinterest Biting the inside of the cheek or lip can cause stomatitis to occur. The most common causes are: trauma from ill-fitting dentures or braces, biting the inside of the cheek, tongue, or lip, and surgery.
What is the fastest way to cure stomatitis?
Anti-inflammatory drugs such as corticosteroids (including prednisone) are the most effective treatment for canker sores, as they will reduce swelling and pain.
Who developed Ervebo?
Manufacturer: Merck Sharp & Dohme Corp.
What is recombinant vesicular stomatitis virus?
Vesicular stomatitis virus (VSV) is a negative-stranded RNA virus and a member of the Rhabdoviridae family, consisting of a simple genome organization encoding five structural proteins: the nucleoprotein (N), phosphoprotein (P), matrix (M), glycoprotein (G) and the RNA-dependent RNA polymerase (L). 7.
How do you treat VS in horses?
No specific treatment is needed. Cleaning ulcers with mild antiseptics may help avoid bacterial infections. To reduce the risk of exposure to the virus, owners may limit pasture time, provide shelters or barns during insect feeding times, and take other steps to reduce animal contact with insects.
How does VSV enter the cell?
VSV enters the cell via the endocytic pathway and subsequently fuses with a cellular membrane within the acidic environment of the endosome. … Many virions that attach to the cell surface are not internalized, and many of the internalized virions appear to be degraded by proteases and other enzymes (Matlin et al., 1982).
What is gag gene?
The gag gene encodes the major structural polyprotein Gag and is all that is necessary and sufficient for the assembly non-infectious and immature viral-like particles. The viral protease is encoded by the pro gene and is responsible for facilitating the maturation of viral particles.
Why are lentiviruses used?
Lentiviral vectors have become particularly attractive for clinical applications due to their ability to more efficiently transduce non-proliferating or slowly proliferating cells, such as CD34 + stem cells.